10.24873/j.rpemd.2022.01.860

Original Research

 

Overall treatment outcome achieved during distal movement of the entire maxillary dentition using mini-implants – A single-center analytical observational clinical study

Resultado geral do tratamento alcançado durante a distalização de toda a dentição maxilar através de mini-implantes – Um estudo clínico observacional analítico de centro único

 

A. Sumathi Felicita¹,* 0000-0003-2002-0140

Lirin Ann Thomas² 0000-0002-1483-5389

¹ Department of Orthodontics and Dentofacial Orthopedics, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

² Department of orthodontics, Faculty of Dentistry, Aimst University, Bedong, Kedah, Malaysia

 

^*Correspondence to:

 

Article history:

Received 18 May 2021

Accepted 22 December 2021

Available online 24 January 2022

 

Abstract

Objectives: To evaluate the overall treatment outcome and undesirable side effects during distal movement of the entire maxillary dentition using mini-implants.

Methods: Seventeen patients older than fifteen years with a mild-to-moderate arch length/tooth size discrepancy were selected. Leveling and aligning were done with 0.018” x 0.025” passive stainless-steel wire in a 0.022 slot. A 1.2 x 8 mm mini-implant was placed bilaterally at the mucogingival junction between the maxillary second premolar and the maxillary first permanent molar. A 200-gram distalizing force was applied. Lateral cephalograms and dental casts were taken prior to distal movement of the maxillary dentition and six months later. Dental casts were assessed using CAD-CAM software. The Wilcoxon signed-rank test was performed.

Results: Intrusion of the entire maxillary arch with decreased lower anterior facial height and facial proportion index was noted. The average extent of molar distalization achieved was 1.7 mm, at the rate of 0.29 mm/month. Distal tipping of the maxillary molars by 3.8° and retraction of the maxillary central incisors by 2.3° were observed. No undesirable side effects occurred, such as incisor flaring, extrusion of the molars, or bite opening. Root resorption was present in most cases.

Conclusions: The overall treatment outcome of distal movement of the maxillary dentition was good. This method is beneficial for patients with mild/moderate arch length/tooth size discrepancy, especially Class II cases, vertical growers, and patients with end on molar relation. Mild root resorption was noted in the maxillary anterior teeth.

Keywords: Clinical study, Distal movement of the maxillary dentition, Mini-implant, Treatment outcome

 

Resumo

Objetivos: Avaliar o resultado geral do tratamento e os efeitos secundários indesejáveis durante a distalização de toda a dentição maxilar através de mini-implantes.

Métodos: Foram selecionados 17 pacientes com idade superior a 15 anos com discrepância dento-alveolar ligeira a moderada. O nivelamento e o alinhamento foram realizados com fio de aço inoxidável de 0.018” x 0.025” numa ranhura de 0.022. Colocou-se um mini-implante de 1,2x8 mm bilateralmente na junção mucogengival entre o segundo pré-molar superior e o primeiro molar permanente. Aplicou-se uma força distalizadora de 200 gramas. Foram obtidos cefalogramas laterais e modelos de gesso antes do movimento distal da dentição superior e seis meses depois. Os modelos de gesso foram avaliados com um software CAD-CAM. Os dados foram analisados estatisticamente com o teste de Wilcoxon.

Resultados: Observou-se intrusão de toda a arcada superior com diminuição da altura facial ântero-inferior e do índice de proporção facial. A extensão média de distalização molar alcançada foi de 1,7 mm, a uma taxa de 0,29 mm/mês. Verificou-se inclinação distal dos molares superiores em 3,8° e retração dos incisivos centrais superiores em 2,3°. Não ocorreram efeitos secundários indesejáveis, como alargamento dos incisivos, extrusão dos molares ou abertura da mordida. Detetou-se reabsorção radicular na maioria dos casos.

Conclusões: O resultado geral do tratamento de distalização da dentição superior foi bom. Este método é benéfico em pacientes com discrepância dento-alveolar ligeira a moderada, especialmente casos de Classe II, padrões verticais e pacientes com relação terminal no molar. Detetou-se uma ligeira reabsorção radicular nos dentes anteriores superiores.

Palavras-chave: Estudo clínico, Movimento distal da dentição Maxilar, Mini-implante, Resultado do tratamento

 

Introduction

Although the mini-implant has been used as an absolute anchorage during space closure to treat malocclusion with a high-anchorage demand,^{1 - 5} distal movement of the entire dentition with mini-implants has not been studied extensively. The distal movement of the maxillary dentition using mini-implants involves retraction of all the teeth in the maxillary or mandibular arch and may be a preferred method for retraction of anterior teeth in patients with mild arch length/tooth size discrepancy as compared to inter-proximal reduction or second premolar extraction.

A thorough and systematic search in all the existing databases did not reveal any well-formulated clinical trial on the distal movement of the maxillary dentition using mini-implants, especially on a thicker arch wire. Although several articles describe molar distalization with an implant-supported system,^{6 - 11} few study the distal movement of the maxillary dentition using mini-implants.^{12 - 14} Earlier studies on the distal movement of the maxillary dentition^{12 - 14} evaluated it on 0.016” x 0.022” stainless-steel wire in a 0.018” / 0.022” slot and some had an insufficient sample size.¹² Most studies evaluated the effect of this treatment’s mechanics on the molars and incisors, but a comprehensive overview of treatment change was not available.

Therefore, the present study proposed to evaluate the overall treatment outcome and side effects during distal movement of the maxillary dentition using mini-implants on a thicker wire: 0.018” x 0.025” stainless steel in a 0.022” slot. This study is unique as there is no published literature on distalization mechanics using the given arch wire/bracket slot combination.

The objective was to evaluate treatment change in the sagittal, vertical, and transverse dimensions and the undesirable side effects produced by distal movement of the maxillary dentition using buccal mini-implants on 0.018” x 0.025” stainless-steel wire in a 0.022” slot in the sagittal, vertical, and transverse dimensions.

Material and methods

This study was approved by the scientific review board and further reviewed by the ethical board. The sample consisted of 17 patients meeting the inclusion and exclusion criteria (Table 1). The inclusion criteria were: more than 15 years of age; mild-to-moderate arch length/tooth size discrepancy; full complement of teeth excluding the third molars; initial leveling and aligning phase of orthodontic treatment; no history of temporomandibular joint problems. The exclusion criteria were: <15 years of age; gross vertical discrepancy; severe skeletal Class II or Class III malocclusion; medically compromised; periodontally compromised; previous orthodontic treatment.

 

Table 1. Baseline data of the participants

 

Based on earlier literature,¹⁴ the power was calculated to be 97% for 16 patients to evaluate the amount of distal movement of the maxillary first permanent molar and the treatment outcome. The effect size was 0.934. After a detailed explanation of the procedure, written informed consent to perform the treatment and publish the images and the relevant findings of the study was obtained from all patients who matched the inclusion criteria and agreed to participate. Eighteen patients were selected for the study to compensate for dropouts. One patient dropped out during the study due to various reasons.

Of the 17 patients, six had an Angle’s Class I molar relation, five had an end on molar relation, and six had an end on one side and an Angle’s Class I on the other side on a Class I skeletal base. Since each patient required a different amount of tooth movement, the amount of distalization during six months was evaluated. Patients with an Angle’s Class I molar relation had a concomitant distal movement of the mandibular dentition. Distalization was carried out in both the upper and lower arch in these patients to correct the proclination of the maxillary and mandibular anterior teeth. These cases did not warrant extracting the premolars for gaining space.

Fixed orthodontic treatment using 0.022 MBT prescription (3M Unitek) was initiated for all patients. Leveling and aligning were sequentially carried out up to a 0.018” x 0.025” stainless-steel wire. The arch wire was sequentially changed from 0.016” nickel-titanium wire to 0.016” x 0.22” stainless-steel wire, followed by 0.017” x 0.025” stainless-steel wire and 0.018” x 0.025” stainless-steel wire. Third molars were removed prior to the placement of the mini-implant. Pre-distalization records, namely extraoral and intraoral photographs, study models, and lateral cephalogram (T1), were taken.

The width of the inter-radicular bone between the maxillary second premolar and the maxillary first permanent molar was assessed on the pre-treatment orthopantomogram to determine the feasibility of mini-implant placement. Indigenous mini-implants (1.2 x 8 mm) were placed bilaterally with a stent ¹⁵ at the mucogingival junction between the maxillary second premolar and the maxillary first permanent molar at an angulation of 30°–40° to the long axis of the tooth.

An intraoral peri-apical radiograph was taken to check the mini-implants’ position. A distalizing force of 200 grams was applied bilaterally with pre-calibrated NiTi coil springs placed from the mini-implants to the crimpable hooks between the lateral incisor and the canine on both sides (Figure 1). The patient was reviewed periodically once every 3 weeks for 6 months. Post-distalization records, namely extraoral and intraoral photographs, study models, and lateral cephalogram, were taken (T2).

 

Figure 1. Distalization of the entire maxillary arch with buccal mini-implants placed between the maxillary second premolar and the maxillary first permanent molar.

 

Lateral cephalograms were used to investigate the treatment outcome. The skeletal, dental, and soft-tissue parameters were evaluated (Figures 2 and 3). The facial proportion index16 was calculated using the following formula: (LAFH/TAFH minus UAFH/TAFH) x 100, where LAFH stands for lower anterior facial height, TAFH for total anterior facial height, and UAFH for upper anterior facial height. The extent of distalization of the maxillary first permanent molar was evaluated individually on the right and left sides. A short stainless-steel wire was placed in the auxiliary tube on the left first permanent molar to differentiate it from the right first permanent molar on the lateral cephalogram. This mark was made to precisely measure the extent of distalization of each maxillary first permanent molar and eliminate errors in measurement.

 

Figure 2. Dental parameters evaluated in the study.

 

Figure 3. Skeletal parameters evaluated in the study.

 

Root resorption was evaluated using intraoral periapical radiographs taken before the distalization and six months after. The extent of root resorption was evaluated as follows: 0 = no root resorption; 1 = blunting; 2 = irregular root outline; 3 = apical root resorption, less than 2 mm; 4 = apical root resorption, from 2 mm to one-third of the original root length; 5 = apical root resorption exceeding one-third of the original root length.

The transverse changes in the maxilla were assessed precisely on the dental casts using a CAD-CAM software (CEREC AC with CEREC BLUECAM 4.2 version). Inter-canine (Figure 4), inter-premolar (Figure 5), and inter-molar (Figure 6) widths were measured between the cusp tip of both maxillary canines, the mesial pit of both maxillary first premolars, and the central pit of both maxillary first permanent molars, respectively.

 

Figure 4. Measurement of inter-canine width using CAD-CAM.

 

Figure 5. Measurement of inter-premolar width using CAD-CAM.

 

Figure 6. Measurement of inter-molar width using CAD-CAM.

 

The various parameters were measured and entered in a proforma sheet. The values obtained were subjected to statistical evaluation. The non-parametric Wilcoxon signed-rank test was done to evaluate treatment changes within the group using the SPSS version 20 software as the sample size was small. The level of significance was evaluated at a 95% confidence interval with p≤0.05. One week after the initial evaluation, five radiographs were retraced in each group by the same investigator. The intra-operator error was assessed using Cohen’s kappa coefficient.

Results

The results obtained from the statistical evaluation are in Tables 1-5. There was no intra-operator error (the value of kappa was 0.9).

The mean age of the patients was 21 + 3.1 years in males and 18 + 3.0 years in females. The patients included in the study tended toward skeletal Class II malocclusion and horizontal growth pattern (Table 1).

Skeletal evaluation (Table 2) showed a reduction in the vertical proportions of the face with a statistically significant reduction in the lower anterior facial height and facial proportion index. There was a significant remodeling of the maxillary and mandibular alveolar bone. There was no statistically significant change in the position of the maxillary and mandibular skeletal bases and their relation to each other with no evident change in SNA, SNB, and ANB. No significant change in basal plane angle, mandibular plane, angle of inclination, upper anterior facial height, posterior facial height, total facial height, gonial angle, and saddle angle was observed.

 

Table 2. Comparison of treatment changes (T1-T2): Skeletal parameters

b. Based on positive ranks

c. Based on negative ranks

d. The sum of negative ranks equals the sum of positive ranks

* Statistically significant

 

Dental evaluation (Table 3) showed significant intrusion of the entire maxillary dentition. There was a significant retraction of the maxillary anterior teeth. Significant distal tipping of the maxillary first permanent molar by 3.88° was noted. The right and left maxillary first permanent molars were evaluated separately to determine the effect of distalization on each molar individually, and their extent of distal movement was almost similar: 1.71 ± 1.687 mm and 1.77 ± 1.48 mm with p=0.004 and p=0.007, respectively. The inter-incisal angle increased. There was a slight clockwise rotation of the maxillary occlusal plane and a marginal decrease in the incisor mandibular plane angle. A significant reduction in overjet occurred.

 

Table 3. Comparison of treatment changes (T1-T2): Dental parameters

b. Based on positive ranks. c. Based on negative ranks. * statistically significant

 

The cephalometric evaluation of soft tissue (Table 4) revealed a significant change in the position of the upper and lower lips. Root resorption was noted in the anterior teeth, with most cases showing irregular root outlines.

 

Table 4. Comparison of treatment changes (T1-T2): Soft Tissue/Root resorption

b. Based on positive ranks. c. Based on negative ranks. * Statistically significant *** Degree of root resorption: 0= no root resorption; 1=blunting, 2= irregular root outline; 3= apical root resorption, less than 2 mm; 4= apical root resorption, from 2 mm to one-third of the original root length; 5= apical root resorption exceeding one-third of original root length.

 

The effect of treatment mechanics on the maxillary arch measured in the transverse plane showed no statistically significant change in inter-canine and inter-premolar width. The inter-molar width increased by 0.38 mm, which was statistically significant (Table 5). The rate of molar distalization achieved on each side was 0.29 mm per month. The incisors retracted 0.39° per month.

 

Table 5. Comparison of treatment changes (T1-T2) Cast Analysis: Transverse parameters

b. Based on positive ranks. c. Based on negative ranks. *Statistically significant

 

Discussion

Distal movement of the maxillary dentition can be considered in cases of borderline skeletal Class II malocclusion with an end on molar relation to achieve a Class I molar relation and concomitantly correct the axial inclination of anterior teeth. It is also indicated in bimaxillary prognathism with mild proclination of the maxillary and mandibular anterior teeth in patients not willing to undergo orthognathic surgery.

Moreover, it can be considered in individuals with Class I malocclusion with mild bidental proclination or mild crowding of the maxillary anterior teeth. However, in these situations, distal movement of the mandibular dentition should be done concomitant with the upper arch. For distal movement of the maxillary dentition to be effective, extraction of third molars is mandatory except when the third molar is deep-seated and located relatively high up in the maxillary tuberosity.¹⁷ Fully erupted maxillary third molars will offer resistance to the distal movement of the maxillary arch, reducing the magnitude of distalization. Only individuals older than 15 years were included in the study to eliminate the effects of growth on the treatment outcome.

The quality of bone may not be satisfactory in younger patients, resulting in failure of the mini-implant.^{18, 19} The mean age of the patients included in the study was 19.06 years + 3.2 months.

The 0.022” x 0.025” slot was used as it is preferred by many orthodontists and has several advantages over the 0.018 X 0.025” slot. Previous literature²⁰ suggests the use of 0.016” x 0.022” stainless-steel arch wire for distalization. However, we selected a thicker arch wire – 0.018” x 0.025” stainless-steel wire in a 0.022” x 0.25” slot, as it will offer better rigidity and torque control than the 0.016” x 0.022” stainless-steel wire.

Despite these precautions, root resorption was noted during treatment. This problem may be minimized by reducing the magnitude of applied force or increasing the time between activation.

The mini-implant site,²¹ diameter,^{22, 23} and placement angulation^{13, 24} were determined considering that the entire maxillary dentition moves upon application of the distalizing force, and the resulting root proximity may result in mini-implant failure. The average success rate of the mini-implant was 82%, and six mini-implants were re-positioned throughout the treatment.

In the present study, the distalizing force was applied from the crimpable hooks placed in the anterior region to the mini-implant, resulting in an oblique force vector with retraction and a tendency toward the intrusion of the maxillary anterior teeth. Application of the point of force at the center of resistance of the maxillary dentition at approximately the same height as the mini-implant will result in a direction of force almost parallel to the occlusal plane, preventing rotational forces on the dentition.5 Some may say that the width of the inter-radicular bone on the buccal aspect may be insufficient between the maxillary second premolar and maxillary first permanent molar for distal movement of the maxillary dentition and loosening of the mini-implant may occur due to root proximity. Mild mobility of the mini-implants IS ACCEPTABLE AND may be considered successful if mini-implant mobility and orthodontic forces are periodically monitored.²⁵

Evaluation of the treatment results showed significant retraction of the maxillary anterior teeth with remodeling of the alveolar bone as shown at the superior prosthion. Significant reduction in the lower anterior facial height and facial proportion index was noted and may be due to the significant intrusion of the maxillary premolars and molars with mild autorotation of the mandible. Hence, this appliance system may be beneficial in vertical growers.

Significant distalization of the maxillary first permanent molar with mild distal tipping was seen. There was a mild reduction in the inter-incisal angle, possibly improving post-treatment stability. Reduction in overjet was seen due to improvement in the axial inclination of the maxillary anterior teeth.

The extent of intrusion from anterior to posterior increased gradually, probably due to the posterior location of the mini-implant. If the direction of force application passes through the center of resistance of the maxillary dentition, only intrusion and distalization of dentition will occur. Otherwise, rotational forces should be anticipated.

Evaluation of the soft tissue showed a change in the position of the upper and lower lips at the end of treatment with improvement in profile. In the transverse plane, no significant change in inter-canine and inter-premolar width was noted. Mild expansion of about 0.38 mm was noted in the molar region.

The rate of molar distalization was around 0.29 mm per month and the rate of retraction of maxillary anterior teeth was 0.39°. The results showed no skeletal change, but the patient was evaluated for only six months.

The extent of distalization was comparable with that of Park HS, ²⁰ greater than that of Bechtold et al., ¹² and less than that of Yamada et al..¹⁴ However, the extent of molar intrusion in the current study was greater than the latter study¹⁴ and may be due to a rigid arch wire and a better bracket-arch wire fit. All the above studies had a longer treatment duration than the current study.

Comparison with other conventional methods of molar distalization like the pendulum appliance and the bone-anchored pendulum appliance (BAPA) showed a lesser extent of distalization and distal tipping.^{6 - 8} The distal jet appliance/skeletonized distal jet appliance supported by mini-implant anchorage²⁶ and the first-class appliance showed greater molar distalization but significant premolar anchorage loss.²⁷

Molar distalization with the skeletal anchorage system using bone plates was about 3.3 to 3.5 mm greater with slight distal tipping of teeth.^{7, 8} However, the bone plate placement is a very invasive procedure compared to the mini-implant placement and must be contemplated with care.

Conclusions

The overall treatment outcome of the maxillary dentition distalization on 0.018 x 0.25” stainless-steel wire in a 0.022 slot was good. Mild root resorption was noted in the maxillary anterior teeth. This method can be applied in cases with a mild-to-moderate arch length/tooth size discrepancy, especially Class II cases. It is beneficial in patients who require intrusion of the maxillary dentition, vertical growers requiring a reduction in the lower anterior facial height, and those with end on molar relation.

 

References

¹ Ali Aljhani, Khalid H. Zawawi. The use of mini-implants in en masse retraction for the treatment of bimaxillary dentoalveolar protrusion. Saudi Dent J. 2010;22,35-9.

² Park HS, Kwon TG. Sliding mechanics with microscrewimplant anchorage. Angle Orthod. 2004;74:703-10.

³ Marassi C, Marassi C. Mini-implant assisted anterior retraction. Dental Press J Orthod. 2008;13:57-74.

⁴ Upadhyay M, Yadav S, Nagaraj K, Nanda R. Dentoskeletal and Soft Tissue Effects of Mini-Implants in Class II division 1 Patients. Angle Orthod. 2009;79:240-7.

⁵ Felicita AS. Quantification of intrusive/retraction force and moment generated during en- masse retraction of maxillary anterior teeth using mini-implants: a conceptual approach. Dental Press J Orthod. 2017;22:47-55.

⁶ Escobar SA. Distalization of maxillary molars with the bone-supported pendulum: a clinical study. Am J Orthod Dentofac Orthop. 2007;131:545-9.

⁷ Kircelli BH, Pektaş ZO, Kircelli C. Maxillary molar distalization with a bone-anchored pendulum appliance. Angle Orthod. 2006;76:650-9.

⁸ Nur M, Bayram M, Celikoglu M, Kilkis D, Pampu AA. Effects of maxillary molar distalization with Zygoma-Gear Appliance. Angle Orthod. 2012;82:596-602.

⁹ Oberti G, Villegas C, Ealo M, Palacio JC, Baccetti T. Maxillary molar distalization with the dual-force distalizer supported by mini-implants: a clinical study. Am J Orthod Dentofac Orthop. 2009;135:282-3.

¹⁰ Polat-Ozsoy O, Kircelli BH, Arman-Ozçirpici A, Pektaş ZO, Uçkan S. Pendulum appliances with 2 anchorage designs: conventional anchorage vs bone anchorage. Am J Orthod Dentofac Orthop. 2008;133:339.e9-17.

¹¹ Sar C, Kaya B, Ozsoy O, Özcirpici AA.Comparison of two implant-supported molar distalization systems. AngleOrthod. 2013;83:460-7.

¹² Bechtold TE, Kim JW, Choi TH, Park YC, Lee KJ. Distalization pattern of the maxillary arch depending on the number of orthodontic miniscrews. Angle Orthod. 2013;83:266-73.

¹³ Park H-S, Jeong SH, Kwon OW. Factors affecting the clinical success of screw implants used as orthodontic anchorage. Am J Orthod Dentofac Orthop. 2006;130:18-25.

¹⁴ Yamada K, Kuroda S, Deguchi T, Takano-Yamamoto T, Yamashiro T. Distal movement of maxillary molars using miniscrew anchorage in the buccal interradicular region. Angle Orthod. 2009;79:78-84.

¹⁵ Felicita AS. A simple three-dimensional stent for proper placement of mini-implant. Prog Orthod. 2013;14:45-8.

¹⁶ Opdebeeck H, Bell WH. The short face syndrome. Am J Orthod. 1978;73:499-511.

¹⁷ Kinzinger GS, Fritz UB, Sander FG, Diedrich PR. Efficiency of a pendulum appliance for molar distalization related to second and third molar eruption stage. Am J Orthod Dentofacial Orthop. 2004;125:8-23.

¹⁸ Miyawaki, S. Factors associated with the stability of titanium screws placed in the posterior region for orthodontic anchorage. Am J Orthod Dentofac Orthop. 2003,124:373-8.

¹⁹ Moon CH, Lee D-G, Lee HS, Im JS, Baek SH. Factors associated with the success rate of orthodontic miniscrews placed in the upper and lower posterior buccal region. Angle Orthod. 2008;78:101-6.

²⁰ Park H-S, Lee SK, Kwon OW. Group distal movement of teeth using microscrew implant anchorage. Angle Orthod. 2005;75:602-9.

²¹ Fayed MMS, Pazera P, Katsaros C. Optimal sites for orthodontic mini-implant placement assessed by cone beam computed tomography. Angle Orthod. 2010;80:939-51.

²² Lee YK, Kim JW, Baek SH, Kim TW, Chang YI. Root and bone response to the proximity of a mini-implant under orthodontic loading. Angle Orthod. 2010;80:452-8.

²³ Poggio PM, Incorvati C, Velo S, Carano A. ‘Safe zones’: a guide for miniscrew positioning in the maxillary and mandibular arch. Angle Orthod. 2006;76:191-7.

²⁴ Wilmes B, Su YY, Drescher D. Insertion angle impact on primary stability of orthodontic mini-implants. Angle Orthod. 2008;78:1065-70.

²⁵ Liou EJ, Pai BC, Lin JC. Do miniscrews remain stationary under orthodontic forces? Am J Orthod Dentofac Orthop. 2004;126:42-7.

²⁶ Carano A, Testa M. The distal jet for upper molar distalization. J Clin Orthod. 1996;30:374-80.

²⁷ Fortini A, Lupoli M, Parri M. The First Class Appliance for rapid molar distalization. J Clin Orthod. 1999;33:322-8.

 

^*Corresponding author:

A. Sumathi Felicita

E-mail address: sumifeli@hotmail.com

 

Ethical disclosures

Protection of human and animal subjects. The authors declare that the procedures followed were in accordance with the regulations of the relevant clinical research ethics committee and with those of the Code of Ethics of the World Medical Association (Declaration of Helsinki).

Confidentiality of data. The authors declare that they have followed their work center protocols on access to patient data and for its publication.

Right to privacy and informed consent. The authors have obtained the written informed consent of the patients or subjects mentioned in the article. The corresponding author is in possession of this document.

 

Conflict of interest

The authors have no conflicts of interest to declare.

 

1646-2890/© 2022 Sociedade Portuguesa de Estomatologia e Medicina Dentária. Published by SPEMD.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).